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Image Search Results
Journal: mSphere
Article Title: Respiratory Syncytial Virus-Induced Oxidative Stress Leads to an Increase in Labile Zinc Pools in Lung Epithelial Cells
doi: 10.1128/mSphere.00447-20
Figure Lengend Snippet: Labile zinc levels increase in primary epithelial cells. (A) SAECs were infected with RSV at an MOI of 3, and cells were stained with ZP-1 at 24 h p.i. and 48 h p.i. (B) Cell viability was determined by staining the cells with the fixable viability dye eFluor780. (C) Nasal epithelial cells isolated from nasopharyngeal aspirates were stained with ZP-1. Labile zinc levels are presented as median fluorescence intensities (MFI). (D) The cell viability of NPA specimens was determined by staining the cells with the fixable viability dye eFluor780. There were 10 controls and 7 cases. (E) A549 cells were infected with RSV and DENV at MOIs of 0.3 and 5, respectively. Cells were stained with Annexin-V and eFluor780 dye at 24 h p.i. and 48 h p.i. Cell populations represent different phases: healthy, early apoptosis, late apoptosis, and necrosis. Annexin-V signal was detected in FITC filter and eFluor-780 signal was detected in APC-Cy-7 filter. Data are from at least two independent experiments. *, P < 0.05; **, P < 0.01; ns, differences were not significant.
Article Snippet: Cells were incubated for 30 min at 37°C in a CO 2 incubator and mixed every 10 min. After 30 min,
Techniques: Infection, Staining, Isolation, Fluorescence